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e coli c321 δa bacteria  (Addgene inc)


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    Structured Review

    Addgene inc e coli c321 δa bacteria
    E Coli C321 δa Bacteria, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+c321+%CE%B4a/C321%2E%CE%94A+(Bacterial+strain+%2348998)/pmc13044316-703-10-19
    Average 93 stars, based on 32 article reviews
    e coli c321 δa bacteria - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Generation and Characterization of Functional Phosphoserine Incorporated Neuronal Nitric Oxide Synthase Holoenzyme
    Article Snippet: .. To examine the performance and compatibility of the phosphoserine incorporation plasmid, E. coli C321.ΔA was co-transformed with pJH-rnNOS or pJH-rnNOS S1412TAG with SepOTSλ (Addgene # 68292). ..

    Article Title: Efficiently Enantioselective Hydrogenation Photosynthesis of ( R )-1-[3,5-Bis(trifluoromethyl)phenyl] ethanol over a CLEs-TiO 2 Bioinorganic Hybrid Materials.
    Article Snippet: Engineering of biological pathways with man-made materials provides inspiring blueprints for sustainable drug production. (R)-1-[3,5-Bis(trifluoromethyl)phenyl]ethanol [(R)-3,5-BTPE], as an important artificial chiral intermediate for complicated pharmaceutical drugs and biologically active molecules, is often synthesized through a hydrogenation reaction of 3,5-bis(trifluoromethyl)acetophenone (3,5-BTAP), in which enantioselectivity and sufficient active hydrogen are the key to restricting the reaction.. In this work, a biohybrid photocatalytic hydrogenation system based on an artificial cross-linked enzymes (CLEs)-TiO2-Cp*Rh(bpy) photoenzyme is developed through a bottom-up engineering strategy.. Here, TiO2 nanotubes in the presence of Cp*Rh(bpy) are used to transform NADP to NADPH during the formation of chiral alcohol intermediates from the catalytic reduction of a ketone substrate by alcohol dehydrogenase CLEs.

    other:

    Article Title: Comparative Analyses of the Transcriptome and Proteome of Escherichia coli C321.△A and Further Improving Its Noncanonical Amino Acids Containing Protein Expression Ability by Integration of T7 RNA Polymerase
    Article Snippet: E. coli C321.ΔA , E. coli MG1655Δ ( ybhB-bioAB )::[λcI857 N( croea59 ):: tetR-bla ] Δ prfA Δ mutS :: zeoR ; all 321 TAG codons changed to TAA , Addgene (ID: 48998).

    Expressing:

    Article Title: Efficiently Enantioselective Hydrogenation Photosynthesis of ( R )-1-[3,5-Bis(trifluoromethyl)phenyl] ethanol over a CLEs-TiO 2 Bioinorganic Hybrid Materials.
    Article Snippet: Engineering of biological pathways with man-made materials provides inspiring blueprints for sustainable drug production. (R)-1-[3,5-Bis(trifluoromethyl)phenyl]ethanol [(R)-3,5-BTPE], as an important artificial chiral intermediate for complicated pharmaceutical drugs and biologically active molecules, is often synthesized through a hydrogenation reaction of 3,5-bis(trifluoromethyl)acetophenone (3,5-BTAP), in which enantioselectivity and sufficient active hydrogen are the key to restricting the reaction.. In this work, a biohybrid photocatalytic hydrogenation system based on an artificial cross-linked enzymes (CLEs)-TiO2-Cp*Rh(bpy) photoenzyme is developed through a bottom-up engineering strategy.. Here, TiO2 nanotubes in the presence of Cp*Rh(bpy) are used to transform NADP to NADPH during the formation of chiral alcohol intermediates from the catalytic reduction of a ketone substrate by alcohol dehydrogenase CLEs.

    Mutagenesis:

    Article Title: Efficiently Enantioselective Hydrogenation Photosynthesis of ( R )-1-[3,5-Bis(trifluoromethyl)phenyl] ethanol over a CLEs-TiO 2 Bioinorganic Hybrid Materials.
    Article Snippet: Engineering of biological pathways with man-made materials provides inspiring blueprints for sustainable drug production. (R)-1-[3,5-Bis(trifluoromethyl)phenyl]ethanol [(R)-3,5-BTPE], as an important artificial chiral intermediate for complicated pharmaceutical drugs and biologically active molecules, is often synthesized through a hydrogenation reaction of 3,5-bis(trifluoromethyl)acetophenone (3,5-BTAP), in which enantioselectivity and sufficient active hydrogen are the key to restricting the reaction.. In this work, a biohybrid photocatalytic hydrogenation system based on an artificial cross-linked enzymes (CLEs)-TiO2-Cp*Rh(bpy) photoenzyme is developed through a bottom-up engineering strategy.. Here, TiO2 nanotubes in the presence of Cp*Rh(bpy) are used to transform NADP to NADPH during the formation of chiral alcohol intermediates from the catalytic reduction of a ketone substrate by alcohol dehydrogenase CLEs.



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    Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the <t>E.</t> <t>coli</t> essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.
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    Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the <t>E.</t> <t>coli</t> essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.
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    Addgene inc e coli c321 δa catalog 49018 strain
    Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the <t>E.</t> <t>coli</t> essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.
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    Addgene inc e coli c321 δa
    Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the <t>E.</t> <t>coli</t> essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.
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    Average 93 stars, based on 1 article reviews
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    Image Search Results


    Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the E. coli essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.

    Journal: Life

    Article Title: A Programmable Finite-Replicated Organism Framework for Balanced Safety and Functionality

    doi: 10.3390/life15091381

    Figure Lengend Snippet: Escape frequency analysis of each suicide module and finite replication dynamics of SerS.F213 FROs. ( a ) Suicide module design schematic featuring dual TAG insertions in essential genes. With the gene editing strategy, the E. coli essential genes ( dnaA , murG , and serS ) were inserted with two TAGs, forming the suicide module (Suicide Module A). ( b ) Quantitative escape frequency measurement expressed as CFU ratio ±Cl2Y supplementation (Mean ± SD, N = 7 biological replicates). Escape frequency was quantified as the ratio of escape mutant colony-forming units (CFUs) to total viable CFUs. ( c ) Engineering strategy for SerS.F213 FROs through rescue module insertion. Two plasmids expressing the ncAA orthogonal translation system and storage protein RFP carrying eight TAGs were constructed. When the exogenous supply of ncAA is interrupted, the storage protein RFP with TAGs degrades and releases the expression of genes necessary for ncAA supply. ( d ) The finite replication characteristics of SerS.F213 FROs. Left panel: growth generation quantification (top) and generational distribution (bottom). The SerS.F213-engineered FROs demonstrated finite proliferation, sustaining four generations of growth before growth arrest, while rescue-module-deficient controls (NC) showed unrestricted proliferation (mean ± SD; **** p < 0.0001, two-tailed Student t -test). Right panel: Microscopic imaging demonstrates finite replication phenotypes in serS -modified FRO. The bacterial growth was observed and filmed every 0, 1, 3, 6, 9, and 12 h.

    Article Snippet: The E. coli C321.ΔA exp strain (Addgene #87359) (Lajoie MJ, 2013) was cultured in Luria–Bertani medium (10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract) supplemented with antibiotics: ampicillin (100 μg/mL), kanamycin (50 μg/mL), chloramphenicol (25 μg/mL), and spectinomycin (50 μg/mL).

    Techniques: Mutagenesis, Expressing, Construct, Two Tailed Test, Imaging, Modification